Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Braz. J. Vet. Res. Anim. Sci. (3) Autor GARCIA,José Fernando (3) D’ÁVILA ASSUMPÇÃO,Mayra Elena Ortiz (1) HEINEMANN,Marcos Bryan (1) HIGA,Zenaide Maria Moraes (1) HO,Linda Lee (1) MELLO,Marco Roberto Bourg de (1) NUNES,Cáris Maroni (1) PANTANO,Thais (1) PANTANO,Thaís (1) Richtzenhain,Leonardo José (1) VASCONCELLOS,Silvio Arruda (1) VISINTIN,José Antonio (1) VISINTIN,José Antônio (1) Mais... Palavra-chave Cryopreservation (2) Bovine (1) Embryo (1) Embryos (1) Ethylene glycol (1) Glycerol (1) Leptospira (1) Leptospirosis (1) Mice (1) Polimerase Chain Reaction (1) Propanediols (1) Royal jelly (1) Semen (1) Sucrose (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2000 (2) 1999 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly Braz. J. Vet. Res. Anim. Sci. VISINTIN,José Antônio; GARCIA,José Fernando; PANTANO,Thais; D’ÁVILA ASSUMPÇÃO,Mayra Elena Ortiz. Compacted mouse morulae were frozen at 0.3ºC/min. or 0.5ºC/min. from -6ºC to -24ºC or -32ºC in 10% of glycerol plus different sucrose concentrations with or without 0.1% of honeybee royal jelly. Embryos were thawed in water bath at 22ºC for 20 seconds and cryoprotectant dilution was done in three steps. Embryos were cultured in Whitten’s medium for 24, 48 and 72 hours at 37ºC, 5% of CO2 and 100% of humidity. The in vitro development ranged from 56.6% to 100% after 72 hours. Expanded blastocysts were transferred to pseudopregnant recipients on the third day of the estrous cycle. Viable fetuses rates for embryos frozen to -24 or -32ºC at 0.3ºC/minute in 10% glycerol + 10% sucrose, 10% glycerol + 10% sucrose + 0.1% honeybee royal jelly, 10% glycerol + 0.1%... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mice; Embryo; Cryopreservation; Sucrose; Royal jelly. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000400009 Detection of Leptospira spp. from pure cultures and from experimentally contaminated bovine semen by polymerase chain reaction Braz. J. Vet. Res. Anim. Sci. HEINEMANN,Marcos Bryan; GARCIA,José Fernando; NUNES,Cáris Maroni; HIGA,Zenaide Maria Moraes; VASCONCELLOS,Silvio Arruda; Richtzenhain,Leonardo José. Due to the high importance of the venereal transmission of bovine leptospirosis, this study aimed to test the ability of PCR to detect Leptospira interrogans serovar hardjo DNA in experimentally contaminated bovine semen. Employing primers directed to the 16S rRNA gene, 10 bacteria/ml of semen could be detected by PCR. Results achieved in this work show that PCR can have a great potential to detect Leptospira spp. in insemination centers. Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Leptospira; Semen; Polimerase Chain Reaction; Bovine. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000100002 Effects of cryoprotectant and plunging temperature in liquid nitrogen on the in vitro and in vivo development of murine morulae Braz. J. Vet. Res. Anim. Sci. PANTANO,Thaís; MELLO,Marco Roberto Bourg de; GARCIA,José Fernando; HO,Linda Lee; VISINTIN,José Antonio. The effects of plunging temperature in liquid nitrogen and cryoprotectant dilution methods were evaluated for compacted mouse morulae frozen in 1.5 M ethylene-glycol (E), 1.5M propylene-glycol (P) or 1.4M glycerol (G). Morulae were equilibrated for 10 minutes in cryoprotectant solution and loaded into 0.25 ml straws with cryoprotectant solution in 3 columns (groups E1, P1, G1) or cryoprotectant in the center and PBS in the lateral columns (E2, P2). Straws were cooled at 0.5ºC/min to -25 or -30ºC and plunged into liquid nitrogen. Straws were thawed in water at 22ºC for 20 seconds. Cryoprotectant was diluted in 3 steps for group G1 and in one step for groups E1 and P1 (direct transfer to PBS + 10% FCS) and E2 and P2 (shaken to mix the 3 columns before... Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryos; Cryopreservation; Glycerol; Ethylene glycol; Propanediols. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000300012 Registros recuperados: 3 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco